Canine Cortisone, CRO ELISA Kit - 96tests

The Canine Cortisone (CRO) ELISA Kit is a competitive enzyme-linked immunosorbent assay specifically designed for the quantitative determination of cortisone in dog serum, plasma, urine, and other biological samples. Cortisone, a biologically active glucocorticoid metabolite of cortisol, plays a vital role in stress response, metabolism, and immune regulation in canines. This assay utilizes a highly specific anti-cortisone antibody pre-coated onto a 96-well microplate. Endogenous cortisone in the sample competes with a horseradish peroxidase (HRP)-labeled cortisone tracer for antibody binding. Following TMB substrate development, the signal intensity measured at 450 nm is inversely proportional to the concentration of cortisone. The kit is optimized for high sensitivity, specificity, and reproducibility, making it suitable for veterinary endocrinology research and diagnostic applications.