Human Prostaglandin F2alpha, PGF2A ELISA Kit - 96tests

The Human PGF2α ELISA Kit is a competitive enzyme-linked immunosorbent assay specifically designed for the quantification of prostaglandin F2α (PGF2α) in human biological matrices such as plasma, serum, urine, and cell culture supernatants. PGF2α, a bioactive lipid derived from arachidonic acid via the cyclooxygenase pathway, plays a critical role in smooth muscle contraction, luteolysis, and inflammatory processes. This assay utilizes a high-affinity anti-PGF2α antibody pre-coated on a 96-well plate, with a PGF2α-HRP conjugate competing with free PGF2α in the sample for antibody binding. The intensity of the colorimetric signal (TMB substrate, measured at 450 nm) is inversely proportional to the concentration of PGF2α. The kit is validated for high sensitivity, specificity, and reproducibility, making it ideal for research in endocrinology, inflammation, and reproductive biology.