GM-CSF ELISA Kit| mouse - 96tests

This high-sensitivity Mouse GM-CSF ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed to detect granulocyte-macrophage colony-stimulating factor (GM-CSF) in mouse serum, plasma, cell culture supernatants, and other biological fluids. The assay utilizes a monoclonal capture antibody specific for murine GM-CSF pre-coated on a 96-well microplate, followed by the addition of samples and a biotinylated detection antibody. Signal amplification is achieved through streptavidin-HRP conjugate binding and TMB substrate conversion, with absorbance measured at 450 nm. The kit provides a broad dynamic range, high specificity with minimal cross-reactivity, and low inter-/intra-assay variability, suitable for profiling GM-CSF-mediated immune responses in preclinical murine models.